Making Precision Cut Tissue Slices
for Ex Vivo Assay Services

Ian MacCloud, Director of Operations, Visikol
Griffin Ferrara, Business Development Lead, Visikol

Original webinar date: September 14, 2021

Webinar objectives:

  • Introduce Visikol, a contract research services company focused on leveraging advanced imaging, 3D cell culture assays and digital pathology to accelerate the drug discovery and development process.
  • Explain the need for in vitro liver models to study livery injury.
  • Demonstrate the standard assay format for creating precision-cut liver slices (PCLS).
  • Explain how the Compresstome VF-310-0Z vibrating microtome helps create uniform tissue slices that can be meaningfully compared between treatments.
  • Go through how to use the Compresstome step-by-step for making PCLS.
  • Discuss how slices are made from normal human liver tissue, diseased human liver tissue, and mouse model liver.

Using electrophysiological methods to examine e-cigarette flavors’ effect on dompamine neuron function

Brandon Henderson, PhD
Assistant Professor
Joan C Edwards School of Medicine at Marshall University

Original webinar date: July 6, 2021Dr. Henderson is an Assistant Professor in the Department of Biomedical Sciences at Marshall University’s Joan C. Edwards School of Medicine. He received a BS (with distinction) in Chemistry at The Ohio State University, where he started research, initially as an Analytical Chemist in the lab of Dr. John Olesik. He went on to receive his PhD in Pharmacology at The Ohio State University in the lab of Dr. Dennis McKay. Afterwards, his professional journey continued his development as a NIDA-NIH Postdoctoral Fellow at the California Institute of Technology in the lab of Dr. Henry Lester. Before starting his faculty position at Marshall University, Dr. Henderson also trained as a visiting postdoctoral fellow at Yale University (under Dr Nii Addy and Dr. Marina Picciotto). In addition to his responsibilities at Marshall University, Dr. Henderson is now one of two co-Chairs for the Basic Science Network in the Society for Research on Nicotine and Tobacco (www.SRNT.org).

The Henderson lab focuses on the role tobacco and vaping flavors play in addiction-related behaviors. Thus far, we have shown that menthol and green apple flavors can enhance nicotine vapor self-administration and do so by directly altering dopamine neurons in the midbrain.

Reflections on a decade of patching in adult brain slices

Johnathan Ting, PHD
Allen Institute

Jonathan T. Ting is an Assistant Investigator at the Allen Institute, where he joined in 2013 to provide electrophysiology expertise for the Human Cell Types program, and to develop functional assays on human ex vivo brain slides. Dr. Ting has more than 15 years of experience in patch clamp electrophysiology. He studied the neural circuitry
basis of psychiatric disorders during his postdoctoral fellowships at Duke University and the McGovern Institute for Brain Research at MIT, and he developed and characterized several transgenic mouse lines now widely employed for nervous system research involving optogenetics.For this webinar, Dr. Ting provides reflections of his experience on a decade of patching adult brain slices. He will:

Webinar objectives:

● Discuss which key steps in the brain slice process is most important and why
● Challenge our conventional beliefs of slicing solutions and methodologies
● Recommend tips and tricks based on his experience and research

Learn how to get higher quality tissue slices

What affects tissue slice quality?
How can you get better slices for IHC or e-phys?

Original webinar date: September 3, 2020

Webinar objectives:

  • To understand and appreciate why tissue slices are used in research, and what slices are used for experimentally.
  • To understand how the common fundamental cutting parameters affect tissue slice quality and health, and learn about possible experimental solutions.
  • To explore and share tips & tricks during the cutting process to improve tissue slice quality.

Learn step-by-step how to section tissue on the cryostat

Step-by-step presentation
Troubleshooting & tips for cryostat cutting
For research and clinical attendees

Original webinar date: September 3, 2020

Webinar objectives:

  • To understand and appreciate why tissue slices are used in research, and what slices are used for experimentally.
  • To understand how the common fundamental cutting parameters affect tissue slice quality and health, and learn about possible experimental solutions.
  • To explore and share tips & tricks during the cutting process to improve tissue slice quality.