Reflections on a decade of patching in adult brain slices

Johnathan Ting, PHD
Allen Institute

Jonathan T. Ting is an Assistant Investigator at the Allen Institute, where he joined in 2013 to provide electrophysiology expertise for the Human Cell Types program, and to develop functional assays on human ex vivo brain slides. Dr. Ting has more than 15 years of experience in patch clamp electrophysiology. He studied the neural circuitry
basis of psychiatric disorders during his postdoctoral fellowships at Duke University and the McGovern Institute for Brain Research at MIT, and he developed and characterized several transgenic mouse lines now widely employed for nervous system research involving optogenetics.For this webinar, Dr. Ting provides reflections of his experience on a decade of patching adult brain slices. He will:

Webinar objectives:

● Discuss which key steps in the brain slice process is most important and why
● Challenge our conventional beliefs of slicing solutions and methodologies
● Recommend tips and tricks based on his experience and research

Learn how to get higher quality tissue slices

What affects tissue slice quality?
How can you get better slices for IHC or e-phys?

Original webinar date: September 3, 2020

Webinar objectives:

  • To understand and appreciate why tissue slices are used in research, and what slices are used for experimentally.
  • To understand how the common fundamental cutting parameters affect tissue slice quality and health, and learn about possible experimental solutions.
  • To explore and share tips & tricks during the cutting process to improve tissue slice quality.

Learn step-by-step how to section tissue on the cryostat

Step-by-step presentation
Troubleshooting & tips for cryostat cutting
For research and clinical attendees

Original webinar date: September 3, 2020

Webinar objectives:

  • To understand and appreciate why tissue slices are used in research, and what slices are used for experimentally.
  • To understand how the common fundamental cutting parameters affect tissue slice quality and health, and learn about possible experimental solutions.
  • To explore and share tips & tricks during the cutting process to improve tissue slice quality.