What is antigen retrieval and why is it important?
Antigen retrieval is the method to help unmask antigenic epitopes that may be covered by cross-linked proteins. This process uses buffers and heat to break cross-links, or methylene bridges, between proteins, allowing the antigenic site to be exposed again for antibody binding. Therefore, antigen retrieval is a method completed before immunohistochemistry or in-situ hybridization staining, in order to maximize your signal.
- Heat-induced epitope retrieval (HIER)
- Proteolytic-induced epitope retrieval (PIER)
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