As the number of Compresstome® vibrating microtome users expand around the world, we would like to showcase some of their research work with you! This week, we feature the research of Professor Daniel Madison from Stanford University.
The Compresstome® Vibrating Microtome in the Madison Lab:
The Madison lab prepares slices of rodent hippocampus and performs whole-cell ‘patch’ electrophysiology recordings from neurons in those slices. In many cases, team members label those neurons by injecting fluorescent markers into them through the recording electrodes, or post-stain them with anti-bodies. It is their aim to correlate the physiological and anatomical properties of these neurons to test specific hypotheses about neuronal function and synaptic plasticity. In a current series of experiments, the Madison lab is making slices from mouse neocortex using the Compresstome® VF-300-0Z vibrating microtome to study the properties of myelin on inhibitory interneurons
“We have only been using the new Compresstome for a few weeks, but have noted an immediate improvement in the quality of our brain slices,” reports Dr. Madison. “In particular, the slices have ‘cleaner’ surfaces and the neurons seem healthier.”
Lab member Dr. Marianna Kiraly preparing mouse hippocampal slices on our Compresstome.
A photograph of a TV screen on Dr. Kiraly’s electrophysiological rig of the surface of the Compresstome®-prepared slices. The lab uses these TV images to target neurons for the electrophysiological electrodes.
A volume reconstruction image of a mouse hippocampal slice, taken from a YFP (yellow fluorescent protein) H-line mouse (green cells), also immunostained for DAPI (blue) and synaptophysin (red). Slice and photo credit: Dr. Ricardo Valenzuela, a former lab member.
To find out more about the Madison Lab at Stanford, visit: https://web.stanford.edu/~madison/madlab/
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