Compresstome® Cutting Parameters

Tissue or SpecimenAdvance
(Speed)
OscillationSuggested
Agarose %
Lung:
  • Precision-cut lung slices
2-33-42%
Any fixed tissues1.5-23-42%
Brain: live
  • Electrophysiology
  • imaging
1.5-23-41.8-2%
Organotypic slices:
Tumor research Brain culture slices
2-34-52%
Retina (fixed and acute slices)
* Infuse with agarose or gelatin
to replace vitreous humor
1.5-25-62.5%
Kidney (acute slices)3-45-62.5%
Liver (acute slices)3-45-62.5%
Cardiac (fixed and acute slices)
* Infuse with agarose or gelatin
to fill up atria/ventricles
1.5-25-62.5%
Gut (fixed and acute slices)3-43-42.5%
Plants: Leaves3-44-52%
Plants: Stems3-44-52%
Plants: Seeds6-82-32.5%
Skin (Epidermis)2-35-62.5%
Muscle (fixed and acute slices)2-34-62.5%
Adipose (fixed and acute slices)4-55-61.8%
Lymph node (fixed and acute slices)
* Use 3% agarose for more fibrous tissues
1.5-25-62.5%
Spleen (fixed and acute slices)3-46-72%