Immunohistochemistry
The Compresstome™ VF-700 is specially designed for cutting fixed tissue slices for floating section immunohistochemistry. Because of its many advantages, it is the machine of choice for floating section studies. The Compresstome™ VF-700 can cut slices as thin as 10 microns, equivalent in thickness to 8 microns of dehydrated paraffin-embeded slices.
Advantages over paraffin microtome
Cutting with the Compresstome™ microtomes requires 3 simple steps. Fix the tissue, embed in agarose, and cut.
Save Time: It saves you 80% of the time.
Saves material: You can skip 6 procedures: no dehydration/hydration, don't need to deal with waxes, or with antigen retrieval.
Better data: Less treatment means higher quality and more dependable data.
Slices in under an hour: Combined with ultra-rapid microwave fixation, the VF-700 can produce histology slices for surgical pathology within an hour.
Advantages over cryostat
Simpler Protocol
There are only three steps for the Compresstome™ protocol --tissue fixation, tissue slicing, and free floating staining. No over night sucrose incubation, no OCT compound embedding, no liquid nitrogen freezing. This saves you time and money.
Stronger signals
The Compresstome™ eliminates the freeze-thaw step(s) that can lead to degradation in proteins and their antigenicities. This reduces false negative results for low level signals.
Better histology image
There is no ice crystal formation with the Compresstome™ slice. Cellular structure and histology image are better preserved. Further EM study is possible.
Wider cutting window
The cyrostat can slice tissues between 4 to 40 microns. For experiments requiring thicker slices, the cryostat slices cracks easily. The Compresstome™ can cut slice thickness from 8 micron and up, with no upper thickness limit.
Versatility
Compresstome™ can cut fresh tissue slices for tissue culture, or for brain slice recording.
Convenient
The footprint of the Compresstome™ is very small. It can be easily moved around from counter to counter.
Affordable
The total cost for the Compresstome™ is only a small fraction of that of a cryostat. The slicer itself is much cheaper. The savings on material cost and time is even bigger.